Journal: Oncology Reports

Article Title: GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling

doi: 10.3892/or.2023.8486

Figure Lengend Snippet: GBP2 regulates PD-L1 expression at mRNA and protein levels. (A) GBP2 expression in HK-2, Caki-1, Caki-2, 786-O, and ACHN was detected using western blotting. (B) PD-L1 and GBP2 mRNA expression were detected by RT-qPCR with shNS and shGBP2#1 in Caki-1 and 786-O cell lines. (C) PD-L1 and GBP2 mRNA expression were detected by RT-qPCR with vector and GBP2 OE in Caki-1 and 786-O. (D) PD-L1 and GBP2 protein expression were detected by western blotting with shNS and shGBP2#1 in Caki-1 and 786-O cell lines. (E) PD-L1 and GBP2 protein levels were detected by western blotting with vector and GBP2 OE in Caki-1 and 786-O cell lines. Data are expressed as the mean ± SD of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. GBP 2, guanylate-binding protein 2; PD-L1, programmed death-ligand 1; RT-qPCR, reverse transcription-quantitative PCR; OE, overexpression; sh-, short hairpin.

Article Snippet: A human proximal tubular epithelial cell line (HK2), along with human Caki-2, and Caki-1 cell lines were purchased from iCell Bioscience, Inc.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation, Binding Assay, Real-time Polymerase Chain Reaction, Over Expression

Journal: Oxidative Medicine and Cellular Longevity

Article Title: A Decade of Research on Coffee as an Anticarcinogenic Beverage

doi: 10.1155/2021/4420479

Figure Lengend Snippet: In vitro studies in cell lines. Effects of different types of coffee on a broad spectrum of human cancer cell lines.

Article Snippet: Makino 2021 [ ] , ACHN Caki-1 , Kidney , Arabica or Robusta Coffee Japan , Roasted , Uninformed , Antiproliferative, antimigratory, apoptosis.

Techniques: In Vitro, Antioxidant Activity Assay, Activity Assay